시호의 뿌리현탁배양에서 직접 체세포배 발생 및 식물체재분화

• 영문명: Direct somatic embryogenesis and plant regeneration from root suspension culture of Bupleurum falcatum L.
• 발행기관: 한국육종학회
• 저자명: 박철호(Cheol Ho Park) 유창연(Chang yeon Yu) 김동욱(Dong Wook Kim) 서정식(Jeong Sik Seo) 안상득(Sang Deuk Ahn) 장병호(Byung Ho Chang)
• 간행물 정보: 『한국육종학회지』Vol.26 No.1, 41~45쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1994.03.30

영문 초록

To improve an efficiency of in vitro propagation of Bupleurum falcatum L., direct somatic embryogenesis and plant regeneration have been attempted in suspension culture of roots differentiated from leaf-derived callus. Rooting from callus suspension culture was better in high concentration than in low concentration of sucrose. Eighteen somatic embryos per 1cm long root segment were induced in MS liquid medium containing 0.5㎎/ℓ 2,4-D. As the concentration of 2,4-D increased, the frequency of somatic embryos at globular stage was higher. A number of somatic embryos at cotyledonary stage were observed in the media supplemented with 0.1-0.5㎎/ℓ 2,4-D. Somatic embryos in the aggregates (somatic embryo clumps) were most frequently found in the medium containing 0.1㎎/ℓ 2,4-D, of which half were consist of 6 to 10 somatic embryos. The frequency of plant regeneration from somatic embryos was highest in 1/4 strength MS medium:11.7 % and 62.5% respectively from somatic embryos at torpedo and cotyledonary stages.

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키워드

Root suspension culture Somatic embryogenesis Plant regeneration Bupleurum falcatum L.