無窮花의 成熟胚에서 體細胞胚 發生과 植物體 再分化

• 영문명: Somatic embryogenesis and plant regeneration from mature zygotic embryos of Hibiscus syriacus L.
• 발행기관: 한국육종학회
• 저자명: Yong Wook Kim(金勇旭) Suk Koo Lee(李錫求) Bong Choon Lee(李逢春) Jae Soon Lee(李載順) Suk Sung Jang(張錫成)
• 간행물 정보: 『한국육종학회지』Vol.24 No.1, 28~34쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1992.03.30

영문 초록

Somatic embryogenesis and plant regeneration were obtained from mature embryos in Hibiscus syriacus L. ‘Bulsae’. Embryos were cultured on modified Murashige and Skoog(MS) or Woody Plant Medium(WPM) supplemented with 1,000mg/ℓ glutamine, 50mg/ℓ glycine and 50mg/ℓ serine, BAP(0 or 1.0mg/ℓ and 2,4-D(0.2~2.0mg/ℓ) in darkness. After 2 months, inoculated explants were transferred to MS medium without growth regulators and additives ; thereafter somatic embryos in various developmental stages were generated. When somatic embryos were transferred to half-strength MS medium containing 1.0mg/ℓ GA₃ and 1.0mg/ℓ ABA, they matured, germinated and produced plantlets with viable shoots and primary roots. Regenerated plantlets were hardened in a perlite:peatmoss(1:1, v/v) potting soil in culture room(25±1℃, 16/8 hour photoperiod) for 4 weeks, then transferred to greenhouse.

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키워드

Hibiscus syriacus L. ‘Bulsae’ Somatic embryogenesis Plant regeneration