無窮花 原形質體 融合에 의한 體細胞 雜種 誘起 Ⅰ. 原形質體 分離에 있어 關聯要因調査

• 영문명: Somatic hybridization by protoplast fusion in hibiscus syriacus and H. rosa-sinensis Ⅰ. Factors affected to protoplast isolation
• 발행기관: 한국육종학회
• 저자명: Kee Yoeup Paek(白基燁) Ju Kwang Hwang(黃周光) Sung Kun Jong(鄭丞根) Sang Il Park(朴相一)
• 간행물 정보: 『한국육종학회지』Vol.21 No.2, 31~38쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1989.06.30

영문 초록

Mesophyll and callus tissues of Hibiscus syriacus and H. rosa-sinensis obtained through in vitro culture of immature embryos were used for protoplast isolation, and several factors influencing the yield were investigated in this experiment. Protoplast isolation from mesophyll tissues required preplasmolysis in CPW 13M medium for 3 or 5 hours under dark conditions. Treatment of mesophyll tissues to 1.0mg/ℓ 2,4-D solution for 12 hours prior to preplasmolysis increased yield of protoplast in H. syriacus and 3.0mg/ℓ 2,4-D for 24 hrs in H. rosa-sinensis. 0.7M mannitol in CPW medium was the most effective as osmoticum and protoplast yield was increased in the enzyme mixture of 3-5% Cellulase, 0.5% Macerozyme and 0.2% Driselase. Physiological age of mesophyll tissue in vitro influenced significantly on the protoplast yield:young tissue grown for less than 5-week was more productive than 8-week old ones for high protoplast yield. Optimum amount of mesophyll and callus tissues in a definite 5 ml of enzyme mixture was 400 and 500 mg, respectively and optimum digestion time for healthy protoplast yield ranged from 5 to 7 hours.

목차

ABSTRACT

緖言

材料 및 方法

結果 및 考察

摘要

引用文獻