배추×무우의 胚珠培養에 의한 屬間雜種 獲得

• 영문명: Obtaining intergeneric hybrid plant between brassica campestris and raphanus sativus through young ovule culture
• 발행기관: 한국육종학회
• 저자명: Soo Seong Lee(李洙聖) Jong Gyu Woo(禹鍾圭) Hyun Ho Shin(申鉉昊)
• 간행물 정보: 『한국육종학회지』Vol.21 No.1, 52~57쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1989.03.30

영문 초록

Around 10 days old immature ovules of heading Chinese cabbage, Brassica campestris ssp. pekinensis, crossed with large rooting radish, Raphanus sativus, were cultured on the modified B₅ medium supplimented with 0.1mg/ℓ 2.4-D or 0.1mg/ℓ BAP. A part of ovules of both treatments were kept in the dark condition for 7 days at the beginning stage of culture and then maintained in the 16 hours illuminated condition like the others. Plants were induced from each of 4 treatments and a total of 102 plants equivalent to 15.1% were obtained. Slightly more plants were produced from the 2.4-D medium and the 24hrs. dark treatemnt for 7 days. Fifteen of 55 F₁ plants treated with colchicine in the nursery stage and 7 of 45 F₁ plants grown without the colchicine treatment produced F₂ seeds from self flower pollination. A total of 170 of 332 F₂ seeds equivalent to 51.7% were germinated and they grown vigourously without chlorosis on the leaf.

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