Picloram-induced plant regeneration from maize shoot segment

• 발행기관: 한국육종학회
• 저자명: 스테픈 제이 메이자(Stephen J. Mejza) 윌리엄 알 크룰(William R. Krul) Byung Dong Kim(金昞東)
• 간행물 정보: 『한국육종학회지』Vol.20 No.1, 70~77쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1988.03.30

영문 초록

Regeneration-competent cultures of mazie have been established not from immature embryos but from rather recalcitrant seedling segments after induction on picloram-containing Murashige-Skoog (MS) media. Eleven-day old seedling shoots of S-type cytoplasmic male sterile line of the W64A inbred were sliced into 2mm sections and placed on MS media containing 10 uM picloram in the dark for 30 days. This was followed by 16 hours of light per day. After two weeks, regeneration-competent tissue was identified, isolated, and then was either maintained on the initial media for more than ten months, or induced to produce plantlets on media containing no auxin. Concentrations of picloram in the media greater than 100 uM produced embryo-like structures incapable of regenerating plants. Picloram was more potent than 2,4-D for inducing embryo-like structures. Regenerated plants have produced either female or male-female combination inflorescenes and viable seeds when pollinated. The second generation produced both male sterile and male fertile, phenotypically normal plants.

목차

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

摘要

LITERATURE CITED