벼 葯培養에 있어서 低溫處理와 分化植物體의 倍數性

• 영문명: Effects of low temperature treatment on culturability and ploidy level in rice anther culture
• 발행기관: 한국육종학회
• 저자명: Sae Jun Yang(楊世準) Jae Keun Sohn(孫再根) Gun Sik Chung(鄭根植)
• 간행물 정보: 『한국육종학회지』Vol.17 No.4, 38~41쪽, 전체 4쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1985.12.30

영문 초록

This experiment was studied to determine the effects of low temperature treatment on culturability and ploidy level of anther-derived plants in rice anther culture. N₆ basic medium supplemented with 2mg/ℓ of NAA and 1mg/ℓ of Kinetin used for callus induction, 0.2mg/ℓ of IAA and 1mg/ℓ of Kinetin for plant differentiation. Rice panicle with flag leaf sheath was pretreated by low temperature at 10℃ for 15 days. Cold-shocked anthers produced more callus induction and plant differentiation about 3 fold than those without pretreatment. Callus induced from cold-shocked anthers produced more haploid plants and less diploid plants than the callus induced from anthers without pretreatment. This tendency was recognized in a variety as well as some F₁ crosses.

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