油菜heterosis 育種에 있어서 母本繼代, 增殖을 위한 組織培養 硏究 Ⅲ. 莖組織片 培養時 生長調節物質이 多個體 分化에 미치는 影響

• 영문명: Tissue cultural studies on the maintenance and multiplication of parental lines in rape(brassica napus L.) heterosis breeding Ⅲ. Effect of growth regulators on multiple shoots differentiation in stem segment culture of rape by in vitro
• 발행기관: 한국육종학회
• 저자명: Jung Il Lee(李正日) Yong Hwan Park(朴用煥) Young Sim Park(朴英心) Rae Kyeong Park(朴來敬)
• 간행물 정보: 『한국육종학회지』Vol.17 No.2, 54~59쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1985.06.30

영문 초록

The objectives of the study were to induce bud formation and multiple shoot differentiation from rape stem tissue in vitro using various combinations of growth regulators NAA and BA concentrations. The results obtained were summarized as follows : Minimum concentration of NAA was 0.3 ㎎/l for root differentiation from the segment of rape stem. Bud and multiple shoots were differentiated by interaction between BA and NAA below the minimum concentration, when the effective BA concentration was 1.5 to 2.0 ㎎/l for the formation of 13 to 14 buds. Of the stem tissues, upper, middle and lower part tissues produced 16, 15 and 8 buds, respectively. Thus upper part tissue was good in forming the buds. Nine multiple shoots were differentiated from the stem tissue growth at the combination of NAA 0.1 ㎎/l and BA 1.5 - 2.0 ㎎/l. Therefore, multiple shoots can be produced from the upper and middle part tissues on the MS medium added with NAA 0.1 ㎎/l and BA 2.0 ㎎/l. The multiple shoots without roots could be divided into individual shoot and then transfered to vermiculite or root differentiation medium in order to get entire plants for maintenance and multiplication of parental lines in heterosis breeding of rapeseed.

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