An In vitro pollination technique for detecting self-incompatibility in cruciferous crops

• 발행기관: 한국육종학회
• 저자명: S.S. LEE(李洙聖) J.Y. YOON(尹震映) H.M. YOON(尹禾模)
• 간행물 정보: 『한국육종학회지』Vol.14 No.3, 66~71쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1982.12.30

영문 초록

An in vitro pollination procedure was developed as an efficient technique for assaying self-incompatibility of Cruciferous crops. Excised flowers were pollinated in the lab, kept in constant relative humidity chambers at 25℃, and the pollen tubes in the style were counted using fluorescence microscopy after staining with aniline blue the next day. A relative humidity (RH) of 98% was optimum for differentiating self-incompatibility vs. self-compatibility with excellent reproducibility. The number of pollen tubes penetrating to stigma under 98% RH was different from plant to plant and age to age of the flower. However. this variation for flowers of the same plant was highly correlated with seed set. Strongly self-incompatible plants could be selected by selecting for the minimum number of pollen tubes across a wide range of flower age for individual plants. This technique seemed to be widely applicable in the study of self-incompatibility.

목차

ABSTRACT

INTRODUCTION

MATERIALS AND METHODS

RESULTS

DISCUSSION

摘要

LITERATURE CITED