Stepwise verification of bone regeneration using recombinant human bone morphogenetic protein-2 in rat fibula model

• 발행기관: 대한구강악안면외과학회
• 저자명: Jung Woo Nam Hyung Jun Kim
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제43권 제6호, 373~387쪽, 전체 15쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2017.12.30

영문 초록

Objectives: The purpose of this study was to introduce our three experiments on bone morphogenetic protein (BMP) and its carriers performed using the critical sized segmental defect (CSD) model in rat fibula and to investigate development of animal models and carriers for more effective bone regeneration. Materials and Methods: For the experiments, 14, 16, and 24 rats with CSDs on both fibulae were used in Experiments 1, 2, and 3, respectively. BMP-2 with absorbable collagen sponge (ACS) (Experiments 1 and 2), autoclaved autogenous bone (AAB) and fibrin glue (FG) (Experiment 3), and xenogenic bone (Experiment 2) were used in the experimental groups. Radiographic and histomorphological evaluations were performed during the follow-up period of each experiment. Results: Significant new bone formation was commonly observed in all experimental groups using BMP-2 compared to control and xenograft (porcine bone) groups. Although there was some difference based on BMP carrier, regenerated bone volume was typically reduced by remodeling after initially forming excessive bone. Conclusion: BMP-2 demonstrates excellent ability for bone regeneration because of its osteoinductivity, but efficacy can be significantly different depending on its delivery system. ACS and FG showed relatively good bone regeneration capacity, satisfying the essential conditions of localization and release-control when used as BMP carriers. AAB could not provide release-control as a BMP carrier, but its space-maintenance role was remarkable. Carriers and scaffolds that can provide sufficient support to the BMP/carrier complex are necessary for large bone defects, and AAB is thought to be able to act as an effective scaffold. The CSD model of rat fibula is simple and useful for initial estimate of bone regeneration by agents including BMPs.

목차

Abstract

Ⅰ. Introduction

Ⅱ. Materials and Methods

Ⅲ. Results

Ⅳ. Discussion

Ⅴ. Conclusion

Conflict of Interest

ORCID

References

키워드

Bone regeneration Bone morphogenetic proteins Carrier Autoclaved autogenous bone Rat fibula