형질전환 베타카로틴 강화 콩 계통 선발 및 도입유전자 특성 분석

• 영문명: Selection of β-carotene Enhanced Transgenic Soybean Containing Single-copy Transgene and Analysis of Integration Sites
• 발행기관: 한국육종학회
• 저자명: 친양(Yang Qin) 권순종(Soon-Jong Kweon) 정영수(Young-Soo Chung) 하선화(Sun-Hwa Ha) 신공식(Kong-Sik Shin) 임명호(Myung-Ho Lim) 권택(Taek-Ryoun Kwon) 조현석(Hyun-Suk Cho) 박순기(Soon Ki Park) 우희종(Hee-Jong Woo)
• 간행물 정보: 『한국육종학회지』Vol.47 No.2, 111~117쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.06.30

영문 초록

The β-carotene biofortified transgenic soybean was developed recently through Agrobacterium-mediated transformation using the recombinant PAC (Phytoene synthase-2A-Carotene desaturase) gene in Korean soybean (Glycine max L. cv. Kwangan). GM crops prior to use as food or release into the environment required risk assessments to environment and human health in Korea. Generally, transgenic plants containing a copy of T-DNA were used for stable expression of desirable trait gene in risk assessments. Also, information about integration site of T-DNA can be used to test the hypothesis that the inserted DNA does not trigger production of unintended transgenic proteins, or disrupt plant genes, which may cause the transgenic crop to be harmful. As these reasons, we selected four transgenic soybean lines expressing carotenoid biosynthesis genes with a copy of T-DNA by using Southern blot analysis, and analyzed the integration sites of their T-DNA by using flanking sequence analysis. The results showed that, T-DNA of three transgenic soybean lines (7-1-1-1, 9-1-2, 10-10-1) was inserted within intergenic region of the soybean chromosome, while T-DNA of a transgenic soybean line (10-19-1) located exon region of chromosome 13. This data of integration site and flanking sequences is useful for the biosafety assessment and for the identification of the β-carotene biofortified transgenic soybean.

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재료 및 방법

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키워드

Transgenic soybean β-Carotene Integration site Single-copy T-DNA