곡류중 Ochratoxin A의 검색을 위한 면역분석법에 관한 연구

• 영문명: Study on the Analysis Method of Ochratoxin A in Cereals by ELISA Method
• 발행기관: 한국환경보건학회
• 저자명: 김동술 정덕화 이용욱
• 간행물 정보: 『1. 한국환경보건학회지』제20권 제3호, 54~60쪽, 전체 7쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 1994.09.30

영문 초록

We established ELISA method which is more rapid and safe than conventional analytical method, and then analyzed concentration of ochratoxin A in the rices by ELISA method. The results were as follows: 1. Since ochratoxin A molecular does not contain the group for coupling reaction, it was first proposed to have the function of an antigen. As a result of conjugation of ochratoxin A-BSA derivatives, one molecular of BSA was conjugated with 13 moleculars of ochratoxin A. 2. On the basis of established ELISA to apply for immuno analytical method of ochratoxin A, the minimal level of detection by ELISA method was at 0.5 ppb. 3. Rices (42) were collected from homes of Kyoungnam districts through November 1992 to December 1992, as a result of analysis, two of these were positive. Rice samples of R-1 and R-9 represented ochratoxin A levels of 6.0 ng/g and 10 ng/g, respectively.