성견악하선에서 Na⁺, K⁺-activated Adenosine Triphosphatase에 대한 실험적 연구

• 영문명: AN EXPERIMENTAL STUDY ON NA⁺, K⁺-ACTIVATED ADENOSINE TRIPHOSPHATASE IN DOG SUBMAXILLARY GLAND
• 발행기관: 대한구강악안면외과학회
• 저자명: 김지학(Jee Hack Kim) 윤중호(Jung Ho Yoon)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제10권 제2호, 195~204쪽, 전체 10쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1984.12.30

영문 초록

Experimental study of purificating trials and specific activity of Na⁺, K⁺-activated Adenosine Triphosphatase in dog submaxillary gland was done and results were obtained as follow; 1. In homogenate, the specific activity of Na⁺, K⁺-ATPase was 0.63 μmoles/min/㎎ㆍprotein. 2. In microsomal fraction, the specific activity of Na⁺, K⁺-ATPase was 2.79 μmoles/min/㎎ㆍprotein. The enzyme was purified 4.4-fold than in homogenate and recovery rate was 80%. 3. In discontinuous gradient, the specific activity of Na⁺, K⁺-ATPase was 20.41 μmoles/min/㎎ㆍprotein. The enzyme was purified 32.4-fold than in homogenate and recovery rate was 20%. 4. In linear gradient, the specific activity of Na⁺, K⁺-ATPase was the highest at fraction 11, and the density was 1.10~1.12g/㎖ at that. The specific activity was 36.29 μmoles/min/㎎ㆍprotein, and the enzyme was purified 57.6-fold than in homogenate.

목차

Ⅰ. 서론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험성적

Ⅳ. 총괄 및 고안

Ⅴ. 결론

참고문헌

ABSTRACT