CO₂Laser가 백서의 4-nitroquinoline 1-oxide(4NQO) 유도 구개암에 미치는 영향에 관한 실험적 연구

• 영문명: EXPERIMENTAL STUDY ON THE EFFECTS OF CARBON DIOXIDE LASER ON 4-NITROQUINOLINE 1-OXIDE(4NQO) INDUCED PALATAL CARCINOMA OF THE ALBINO RAT
• 발행기관: 대한구강악안면외과학회
• 저자명: 이의웅(Eui Wung Lee) 양성익(Sung Ik Yang)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제11권 제2호, 169~187쪽, 전체 19쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1985.12.30

영문 초록

The carbon dioxide laser has become more available widely in recent years and is now employed in many branches of surgery. It is important to understand the biological effects of CO₂ laser on normal oral mucosa and tumor tissues so that the likely response in patients can be predicted. However, little information is available on the biological effects of CO₂ laser in removing the tumor tissues. The present study was undertaken to investigate the effects of carbon dioxide laser on 4-nitroquinoline 1-oxide (4NQO) induced palatal carcinoma of the albino rats. 113 albino rats, Sprague Dawley strain, 6 week old, were used in this experimental study, divided into control group (15) and experimental group (30) in the first experiment (experimental carcinogenesis), then, normal group (33) and cancer group (35) in the second experiment (Laser irradiation). The first experiment was carried out to produce induced palatal carcinoma by the application of 0.5% 4NQO in propane 1,2-diol on palatal mucosa of the albino rats three times weekly for 30 weeks. The animals of experimental group were sacrificed every other week serially after the application of carcinogen. In the second experiment, CO₂ laser and blade wounds were created on normal palatal mucosa while laser wound on the tumor tissues. All animals were sacrificed serially immediately, 2, 4, 7, 14, 21, 28th day after each wound creation. The palatal mucosa was excised involving the laser or blade wounds. Tissue was examined grossly, light-microscopically and electron-microscopically. The obtained results were as follows. 1. In the experimental carcinogenesis for 4NQO induced palatal carcinoma, mild acanthosis only in 2 to 8th week, marked acanthosis with early acantholysis and epithelial dysplasia in 10 to 22th week and well differentiated squamous cell carcinoma in 24 to 30th week were observed lieht-microscopically. 2. In electron microscopic observation, we observed decrease of desmosomes and widening of intercelluar space in 6 to 10th week of carcinogenesis. Vacuolar degeneration and decrease of tonofilaments in cytoplasm were also seen in 10 to 20th week progressively. Lately, destruction of desmosome, widening of intercellular space, vacuolar degeneration and swelling of mitochondria in cytoplasm, cytoplasmic process extension with absence of basal lamina, irregular patterns of nucleus and cell features, prominent nucleoli were observed in 24 to 30th week of squamous cell carcinoma. 3. In light microscopic observation of the blade wounds in normal palatal mucosa, necrosis, inflammation and granulation tissue formation were seen until 7th day after wound creation but fibrosis and normal connective tissue formation were observed after 1st week, while in the laser wound, more severe necrosis, inflammation and granulation tissue formation were seen continuously until 3rd week, but fibrosis was seen on 3rd week and similar healing findings compared to blade wound were noted on 4th week. Fibrin thrombi formation was also observed variably within blood vessels. 4. In the laser wound of palatal carcinoma, its healing process was similar to that in normal palatal mucosa with extensive necrosis and fibrin thrombi within blood vessels, but the ultrastructural changes of tumor cell were not observed. Specific morphologic changes of the laser wound were extensive tissue necrosis and fibrin thrombi formation within blood vessels of connective tissue mainly, thus delayed healing was prominent in the early stage, but there were no significant differences between laser and blade wound in the later healing process. It was considered that direct dissemination of the tumor cell was probably inhibited due to vascular occlusion by intravascular thromboses during CO₂ laser surgery for malignant tumor

목차

ABSTRACT

Ⅰ. 서론

Ⅱ. 실험재료 및 방법

Ⅲ. 실험성적

Ⅳ. 총괄 및 고찰

Ⅴ. 결론

참고문헌