白鼠 下顎前齒 拔齒窩에 DMBA 및 Tobacco tar 注入時 拔齒創의 發癌性에 關한 實驗的 硏究

• 영문명: Carcinogenic effects of DMBA and tobacco tar in extraction wounds
• 발행기관: 대한구강악안면외과학회
• 저자명: Choi Jin Yong(崔津鏞) Sang Chull Lee(李相喆)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제22권 제2호, 393~408쪽, 전체 16쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1996.04.30

영문 초록

This study was undertaken to observe the carcinogenesis induced by DMBA injection to extraction wound and the effects of the tobacco-tar on that. In order to carry out this experiment, 40 white rats were used and divided into 3 groups, two experimental(1, 2) and one control group. Experimental group 1 was injected into extraction socket of lower incisor tooth with 0.1~0.2ml of DMBA in mineral oil. Experimental group 2 was injected into extraction wound of lower incisor tooth with the mixture of DMBA and 0.5gm of tobacco tar. 4 animals in each experimental group and 2 animals in control group were killed at intervals of 4th, 8th, 10th, and 12th, week. At the time of death, the mandible was dissected, fixed in 10%neutral formalin, decalcified with 5% nitric acid, embedded in paraffin and sectioned 4~6( in thickness. The sections stained with Hematoxylin-Eosin, PAS, and Masson s trichrome. The results of this study were obtained as follows; 1. In DMBA injection group, extraction socket was undertaken inflammatory reaction and filled with connective tissue at 4th week, but at 8th week, fibrosarcoma initiated and dysplastic enlargement in gingiva and minor salivary glands were observable. At 10th-12th week, growth of fibrosarcoma and carcinoma was prominent. 2. In the mixture of DMBA and tobacco tar injection group, the growth of fibrosarcoma and carcinoma was initiated at 4th week and at 10th week, was prominent. Highly-differentiated squamous cell carcinoma in the gingiva and adenocystic carcinoma in the minor salivary glands were observable. At 12th week, malignancy was more prominent. 3. The growth of fibrosarcoma and carcinoma in the area of extraction wound affected the area of posterior teeth, and carcinoma in the gingiva, hyperplasia of cementum and pulp necrosis were observable. 4. The neuromatic growth in the nerve bundle and the chondromatous growth in the new bone tissue were observable. 5. Carcinogenesis induced by the mixture of DMBA and tobacco tar in the extraction wound was more prominent than DMBA alone injection and had a carcinogenic effect on the all surrounding tissue.

목차

Abstract

I. 緖論

II. 實驗材料 및 方法

III. 硏究成績

IV. 總括 및 考察

V. 結論

參考文獻