우골에서 추출한 골형성단백을 이용한 백서 두개골 결손부 치유에 관한 연구

• 영문명: A HISTOLOGIC STUDY ON THE REPAIR OF RAT CALVARIAL CRITICAL SIZE DEFECT WITH BOVINE BONE MORPHOGENETIC PROTEIN (b-BMP)
• 발행기관: 대한구강악안면외과학회
• 저자명: 채윤필(Yun Pil Chae) 이종호(Jong Ho Lee) 김수경(Soo Kyung Kim) 여환호(Hwan Ho Yeo)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제23권 제2호, 290~303쪽, 전체 14쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1997.04.30

영문 초록

Bovine BMP had been partially purified by guanidine HCI extraction and heparin affinity chromatography. It had distinct 3 bands between 20kDa and 30kDa in SDS-polyacrylamide gel electrophoresis. The critical size defect of rat calvaria, 8mm size in diameter, was used to establish osteoinductive activity of BMP and other bone graft materials. Bovine BMP plus IBM(inactivated bone matrix) as a carrier, allogenic demineralized freeze-dried bone (DFDB) and autogenous cortico-cancellous bone were implanted into the 12 rat skull defect of each group in order to evaluate repairing capacity The animals were sacrificed at the week of 2nd, 4th, 6th and 8th after operation The results were as follows 1. In the BMP-IBM complex Implantation group, the defect has been repaired completely by the 8th postoperative week. At 2 week, duster of chondroblast and cartilage appeared around the BMP-coated implant matrix. Calcification began at 6 week. At 8 week, the woven bone changed lamella bone with hematopoietic marrow and it has been bridged the both end of skull defect. 2. The repairing speed and quantity of new bone formation of BMP-IBM complex implant were superior to those of autogenous bone graft and DFDB implant. The defect which was filled with DFDB healed not only by new bone but also by retained implanted bone. The inflammatory and immune reaction of DFDB graft were more apparent than those of BMP implant and autogenous bone graft. 3. It has been assumed that the bovine BMP could be effectively used for the treatment of complicated bone defects in oral and maxillofacial region.

목차

Abstract

I. 서론

II. 연구 재료 및 방법

III. 연구 성적

IV. 총괄 및 고안

V. 결론

참고문헌

키워드

Bone morphogenetic protein BMP Bone regenerating material Critical size defect