Propolis가 마우스에서 종양발생 및 비장세포와 대식세포의 성장에 미치는 영향

• 영문명: EFFECTS OF PROPOLIS ON THE TUMORIGENESIS AND THE GROWTH OF SPLENOCYTES AND MACROPHAGES IN MICE
• 발행기관: 대한구강악안면외과학회
• 저자명: 허홍열(Hong Yeol Huh) 김진수(Chin Soo Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제24권 제2호, 157~164쪽, 전체 8쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 1998.04.30

영문 초록

The propolis, honey bee hive product, is a folk medicine for treating various ailments. Many important pharmaceutical properties have been ascribed to propolis, including anti-inflammatory, antimicrobial, immunormodulatory and carcinostatic activities. The purpose of this study was to examine the effects of ethanol extracted propolis(EEP) on the tumorigenesis and the growth of splenocytes and macrophages in ICR mice. Topical application of 0.2, 2 or mg/ml of EEP on the back of each mice 30 minutes before the application of 7, 12-dimethylbenz (a)anthracene(DMBA) and 12-O-tetradecanoylphorbol-13-acetate(TPA) inhibited the number of tumors per mouse by 61, 75 or 100%, respectively, and tumor size per mouse was decreased by 37, 75 or 100%, respectively. In 3-methylcholanthrene induced tumorigenesis, topical application of same doses of EEP inhibited the number of tumors per mouse by 19, 60 or 90%, and the tumor size per mouse by 58, 85 or 98%, respectively. Oral administration of mice with EEP at 0.2mg or more per day per mouse for 28days increased pulmonary metastases of the B16F10 melanoma cells in ICR mice by 240%, EEP of 3.75ug/ml or more inhibited the growth of ICR mouse spleen cells significantly(p<0.05), but EEP 0.94ug/ml or less did not affects the growth of the spleen cells in vitro. Caffeic acid phenethyl ester(CAPE), which is derived from the propolis, of 0.94ug/ml or less increased the growth of spleen cells, but at the concentations of 3.75ug/ml or more, decreased the growth of spleen cells. EEP of 3.75-15ug/ml increased the growth of mouse peritoneal macrophages, but at the concentration of 60ug/ml, decreased the growth of the macrophages significantly(p<0.01). And also, CAPE of 0.94ug/ml or less did not affects the growth of the macrophages, but at the concentrations of 3.75ug/ml or more, decreased the growth of the macrophages significantly(p<0.01). DDP or CAPE inhibited the nitric oxide production of mouse peritoneal macrophages in concentration-dependent manner regardless of the number of the macrophages in vitro. These results suggest the possibility that the EEP or CAPE might suppress the immune function by the inhibition of growth and activity of spleen cells and macrophages.

목차

Abstract

I. 서론

II. 재료 및 방법

III. 성적

IV. 고찰

V. 요약

참고문헌

키워드

Propolis tumorigenesis immunfunction