HPLC-MS/MS를 이용한 트리클로로에틸렌 대사산물의 다중 분석법 확립

• 영문명: Multiple Determinations of Trichloroethylene Metabolites in a Concurrent Biological Media using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry
• 발행기관: 한국환경보건학회
• 저자명: 안영아(Youngah Ahn) 고영림(Younglim Kho) 이승호(Seungho Lee) 신미연(Mi-yeon Shin) 전중대(Jung Dae Jeon) 김성균(Sungkyoon Kim)
• 간행물 정보: 『1. 한국환경보건학회지』제40권 제2호, 114~126쪽, 전체 13쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2014.04.30

영문 초록

Objectives: We aimed to develop a measurement method of five metabolites of trichloroethylene(TCE) in a concurrent biological sample, e.g., trichloroacetic acid(TCA), dichloroacetic acid(DCA), S-(1,2-dichlorovinyl) glutathione(DCVG), S-(1,2-dichlorovinyl)-L-cysteine(DCVC), and N-Acetyl-S-(1,2-dichlorovinyl)-L-cysteine (NAcDCVC) and to validate the method before application to pharmacokinetic study. Methods: TCE metabolites were simultaneously analyzed using high performance liquid chromatography coupled with electrospray ionization mass spectrometry(HPLC-ESI-MS/MS) with as little as 50 µL of serum and urine. DCA, TCA and NAcDCVC were extracted with diethyl ether, while DCVC and DCVG were extracted by solid phase extraction. This method was validated according to the guidelines for bioanalytical method validation of the Korean National Institute of Toxicological Research. Then, we determined the five metabolites in five strains of mice at 24 hr after exposure to 1 g TCE/kg body weight. Results: The limits of detection for the five metabolites in biological samples ranged from 0.001 to 0.076 nmol/mL, which is comparable to or better than those previously reported. Most calibration curves showed good linearity (R 2 =0.99), and between-batch variation was less than 20% expressing acceptable robustness and reproducibility. Using this method, we found TCA and DCA were detected in all test mice at 24 hr after the oral administration while NAcDCVC and DCVC were detected in some strains, which showed strain-dependent metabolism of TCE. Conclusions: The present method could provide robust and accurate measurements of major key metabolites of TCE in biological media, which allowed concurrent analysis of TCE metabolism for limited amounts of biospecimens.

목차

I. 서 론

II. 재료 및 방법

III. 결 과

IV. 고 찰

V. 결 론

키워드

Biomarkers Exposure HPLC-ESI-MS/MS Metabolites Trichloroethylene