과산화수소 증기 시스템을 이용한 미생물 제독에 관한 연구

• 영문명: A Study on Microorganisms Decontamination Using a Vapor-Phase Hydrogen Peroxide System
• 발행기관: 한국환경보건학회
• 저자명: 김윤기(Yun Ki Kim) 김민철(Min Cheol Kim) 윤성녀(Sung Nyo Yoon) 황현철(Hyun Chul Hwang) 류삼곤(Sam Gon Ryu)
• 간행물 정보: 『1. 한국환경보건학회지』제39권 제3호, 279~288쪽, 전체 10쪽
• 주제분류: 공학 > 환경공학
• 파일형태: PDF
• 발행일자: 2013.06.30

영문 초록

Objectives: Effectiveness and conditions of vapor-phase hydrogen peroxide (VPHP) system on decontamination of Geobacillus stearothermophilus(GS) spores, Escherichia coli (E.coli) and Enterobacteria phage felix01 (felix01) were determined. Methods: The VPHP system was designed to vaporize 35% (w/w) solution of hydrogen peroxide, continuously to inject and withdraw VPHP. The system and VHP 1000ED (Steris) were operated such that dehumidification and conditioning were initiated without samples in the chamber. Then the samples were loaded into and removed. Coupons (glass, anodizing, silicon, viton) with GS spores (1×106 colony forming unit/mL [CFU/mL]), E.coli (1×107 CFU/mL) and felix01 (1×107 plaque forming unit/mL[PFU/mL]), and Biological Indicator (BI) with GS spores (1×106 CFU/mL) on stainless steel coupons were used. The tested samples were sonicated and vortexed, and then were plated for enumeration, followed by incubation at 55oC, 24 hr for GS spores, and at 37 oC, 24 hr for E.coli and felix01. BI analysis in broth culture was only qualitative. Results: The efficacy of the VPHP system on decontamination was almost equivalent to that of VHP 1000ED. The conditions for complete decontamination with the VPHP system was as follows: concentration; 700~450 ppm, relative humidity; approximately 55%, and temperature; 34~32 oC. When comparing the decontamination efficiency among different kinds of coupons, glass was the most effective, however, all kinds of coupons were decontaminated completely after 60 min exposure in both systems. Conclusion: The VPHP system can be recommended as an alternative system for traditional system using ethylene oxide, formaldehyde or chlorine dioxide.

키워드

Biological indicator coupon decontamination dehumidification vapor-phase hydrogen peroxide system