구강암세포주에서의 Tamoxifen의 항암효과

• 영문명: ANTICANCER EFFECT OF TAMOXIFEN IN ORAL CANCER CELL
• 발행기관: 대한구강악안면외과학회
• 저자명: 정재화(Jae-Hwa Jung) 윤필영(Pil-Young Yun) 명훈(Hoon Myoung) 신재일(Jae-Il Shin) 이종호(Jong-Ho Lee) 김명진(Myung-Jin Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제29권 제6호, 365~373쪽, 전체 9쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2003.12.30

영문 초록

Tamoxifen is an selective estrogen receptor antagonist widely used in the management of patients with breast cancer for more than 30 years. It was thought to act primarily through occupying the estrogen receptor sites in ER positive breast cancer cells and directly on cancer cell proper. These inhibitory effects, which have been shown to be independent of the ER, highlight new mechanism of therapeutic action of tamoxifen. The purposes of this study were to identify ER in oral carcinoma cell lines and to evaluate ER independent cytotoxic effect of tamoxifen. KB(SCC), HSC-3(SCC) and A253(ACC) cell line were used and capacity of cell proliferation, apoptosis, in vitro invasion and gelatin zymography were tested. ER expression of each cell line were detected by RT-PCR and immunocytochemistry. Dose dependent inhibition of cell proliferation and inhibition of gelatinolytic activity were observed in all oral carcinoma cell lines and significant difference of apoptotic index were observed in A253 and KB. Tamoxifen inhibited in vitro invasion in all experimental groups. ER expression was detected in KB and A253. These data suggest that tamoxifen may play a role in management of oral carcinoma by independent cytotoxic effect and more advanced research must processed confirming ER-dependent cytotoxicity.

키워드

Tamoxifen Oral carcinoma Apoptosis Anti-cancer effect Estrogen