말초신경 재건을 위한 인회석 박막 코팅 미세공성 신경재생관(nerve conduit)의 개발

• 영문명: DEVELOPMENT OF MICROPOROUS CALCIUM PHOSPHATE COATED NERVE CONDUIT FOR PERIPHERAL NERVE REPAIR
• 발행기관: 대한구강악안면외과학회
• 저자명: 이종호(Jong-Ho Lee) 황순정(Soon-Jeong Hwang) 최원재(Won-Jae Choi) 김성민(Soung-Min Kim) 김남열(Nam-Yeol Kim) 이은진 안강민(Kang-Min Ahn) 명훈(Hoon-Myung) 서병무(Byoung-Moo Seo) 최진영(Jin-Young Choi) 정필훈(Pill-Hoon Choung) 김명진(Myung-Jin Kim) 김현만(Hyun-Man Kim) 박경표(Kyung-Pyo Park) 김중수(Joong-Soo Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제29권 제3호, 151~156쪽, 전체 6쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2003.06.30

영문 초록

This study was performed to develop a useful nerve conduit which provides favorable environment for Schwann cell viability and proliferation. Milipore membrane of 0.45μm pore size was selected because it permits nutritional inflow from the outside of the conduit and prevents from invading the fibrotic tissue into the conduit. The membrane was rolled and sealed to form a conduit of 2mm diameter and 20mm length. To improve the axonal regeneration and to render better environment for endogenous and exogenous Schwann cell behaviour, the microgeometry and surface of conduit was modified by coating with thin film of calcium phosphate. Cellular viability within the conduit and attachment to its wall were assessed with MTT assay and SEM study. Milipore filter conduit showed significantly higher rate of Schwann cell attachment and viability than the culture dish. However, the reverse was true in case of fibroblast. Coating with thin film of low crystalline calcium phosphate made more favorable environment for both cells with minimal change of pore size. These findings means the porous calcium phosphate coated milipore nerve conduit can provide much favorable environment for endogenous Schwann cell proliferation and exogenous ones, which are filled within the conduit for the more advanced strategy of peripheral nerve regeneration, with potential of reducing fibrotic tissue production

키워드

Nerve regeneration Milipore conduits Schwann cells Calcium phosphate coating