골육종세포에 미치는 레이저 조사의 광독성 효과

• 영문명: PHOTOTOXIC EFFECTS OF LOW LEVEL LASER IRRADIATION ON HUMAN OSTEOSARCOMA CELLS
• 발행기관: 대한구강악안면외과학회
• 저자명: 손장호(Jang-Ho Son) 조영철(Young-Chul Cho) 류성호(Sung-Ho Ryu) 김규천(Gyoo-Cheon Kim) 성일용(Iel-Yong Sung) 박봉수(Bong-Soo Park)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제30권 제6호, 509~515쪽, 전체 7쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2004.12.30

영문 초록

Photodynamic therapy(PDT) has advanced to clinical trials for the treatment of a variety of solid tumors and presents an alternative treatment option for tumors resistant to chemo-and/or radio-therapy. PDT is based on the combination of laser light of appropriate wavelength and energy to activate a systemically or locally applied photosensitizer that concentrates preferentially in malignant tissues. In this study, phototoxicity of laser EIT 21 was analysed in human osteosarcoma cell(HOS) and the second objective of this study was to determine the ability of laser EIT 21 to induce apoptosis. This study demonstrated that laser EIT 21 had a phototoxicity to HOS cells. In order to examinate whether cell death was induced by necrosis or apoptosis, variety of techniques which assess apoptosis were used. TUNEL assay showed only a few the positive reaction on condensed nuclei. It is hard to find condensed or fragmented nuclei on HOS cells irradiated with laser EIT 21 in Hemastat and AO/EB stain. By DNA electrophoresis, cells also did not show DNA degradation characteristic of apoptosis with a ladder pattern of DNA fragments. Apoptosis-related factors were analyzed by western blotting. The expression of p53 was constant and cells irradiated with laser did not show the caspase-3 and PARP degradation, therefore we suggest that p53 and caspase-3 are not involved in laserinduced cell death.

키워드

PDT Laser light Apoptosis