BDNF 유전자 이입 슈반세포와 PGA 도관을 이용한 백서 좌골신경 재생에 관한 연구

• 영문명: PERIPHERAL NERVE REGENERATION USING POLYGLYCOLIC ACID CONDUIT AND BRAIN-DERIVED NEUROTROPHIC FACTOR GENE TRANSFECTED SCHWANN CELLS IN RAT SCIATIC NERVE
• 발행기관: 대한구강악안면외과학회
• 저자명: 최원재(Won-Jae Choi) 안강민(Kang-Min Ahn) 고은봉(En-Feng Gao) 신영민(Young-Min Shin) 김윤태(Yoon-Tae Kim) 황순정(Soon-Jeong Hwang) 김남열(Nam-Yeol Kim) 김명진(Myung-Jin Kim) 조승우(Seung-Woo Jo) 김병수(Byung-Soo Kim) 김윤희(Yun-Hee Kim) 김성민(Soung-Min Kim) 오승재(Seung-Jae Oh) 김성준(Sung-Jun Kim) 장정원(Jeong-Won Jahng) 이종호(Jong-Ho Lee)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제30권 제6호, 465~473쪽, 전체 9쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2004.12.30

영문 초록

Purpose : The essential triad for nerve regeneration is nerve conduit, supporting cell and neurotrophic factor. In order to improve the peripheral nerve regeneration, we used polyglycolic acid(PGA) tube and brain-derived neurotrophic factor(BDNF) gene transfected Schwann cells in sciatic nerve defects of SD rat. Materials and methods : Nerve conduits were made with PGA sheet and outer surface was coated with poly(lactic-co-glycolic acid) for mechanical strength and control the resorption rate. The diameter of conduit was 1.8㎜ and the length was 17㎜. Schwann cells were harvested from dorsal root ganglion(DRG) of SD rat aged 1 day. Schwann cells were cultured on the PGA sheet to test the biocompatibility adhesion of Schwann cell. Human BDNF gene was obtained from cDNA library and amplified using PCR. BDNF gene was inserted into E1 deleted region of adenovirus shuttle vector, pAACCMVpARS. BDNF-adenovirus was multiplied in 293 cells and purified. The BDNF-Adenovirus was then infected to the cultured Schwann cells. Left sciatic nerve of SD rat (250g weighing) was exposed and 14㎜ defects were made. After bridging the defect with PGA conduit, culture medium(MEM), Schwann cells or BDNFAdenovirus infected Schwann cells were injected into the lumen of conduit, respectively. 12 weeks after operation, gait analysis for sciatic function index, electrophysiology and histomorphometry was performed. Results : Cultured Schwann cells were well adhered to PGA sheet. Sciatic index of BDNF transfected group was -53.66±13.43 which was the best among three groups. The threshold of compound action potential was between 800 to 1000μA in experimental groups which is about 10 times higher than normal sciatic nerve. Conduction velocity and peak voltage of action potential of BDNF group was the highest among experimental groups. The myelin thickness and axonal density of BDNF group was significantly greater than the other groups. Conclusion : BDNF gene transfected Schwann cells could regenerate the sciatic nerve gap(14㎜) of rat successfully.

키워드

Peripheral nerve regeneration PGA tube BDNF Schwann cell Gene transfection