배양된 구강점막 각화상피세포가 누드마우스 피부 창상 치유에 미치는 효과

• 영문명: THE INDUCTIVE CAPACITY OF PRIMARY CULTURED ORAL MUCOSAL KERATINOCYTES IN SKIN WOUND HEALING OF ATHYMIC NUDE MICE
• 발행기관: 대한구강악안면외과학회
• 저자명: 김현실(Hyun-Sil Kim) 김남희(Nam-Hee Kim) 김진(Jin Kim) 차인호(In-Ho Cha)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제30권 제4호, 308~315쪽, 전체 8쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2004.08.30

영문 초록

Aim: The aim of this study was to investigate the mechanism of promoted skin wound healing in skin defects with primary cultured oral mucosal keratinocytes. Materials and methods: Thirty adult female nude mice weighing 20±2g were used for the experiment. Primary cultured and suspended oral mucosal keratinocytes, labeled with BrdU, were scattered onto 1.5㎝×1.5㎝ sized full thickness skin defects in the experimental group(N=15), and no grafts were placed the control group(N=15). They were sacrificed at 3 days, 1 week and 2 weeks after the treatment respectively. Histological examination of each wounds were performed to review the healing progress on measuring the length from the wound margin to regenerating epithelial front. The role of keratinocytes were assessed by double immunohistochemical staining with Anti-BrdU and Anti-cytokeratin AE1/3. Results: In the experimental group the wound was completely covered with regenerating epithelia in 2 weeks, but partially regenerated in the control group. The immunohistochemical studies unexpectedly reveal that most of regenerating epithelial cells were induced from marginal epithelium of the margin, not from the scattered keratinocytes. Conclusion: We could successfully confirm that graft of primary cultured oral mucosal keratinocytes promotes the regeneration of skin defects.

키워드

Oral mucosal keratinocyte Primary culture Wound healing Tissue engineering