삼차원 배양된 슈반세포 도관을 이용한 말초 신경 재생

• 영문명: PERIPHERAL NERVE REGENERATION USING A THREE-DIMENSIONALLY CULTURED SCHWANN CELL CONDUIT
• 발행기관: 대한구강악안면외과학회
• 저자명: 김성민(Soung-Min Kim) 이종호(Jong-Ho Lee)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제30권 제1호, 1~16쪽, 전체 16쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2004.02.28

영문 초록

The use of artificial nerve conduit containing viable Schwann cells is one of the most promising strategies to repair the peripheral nerve injury. To fabricate an effective nerve conduit whose microstructure and internal environment are more favorable in the nerve regeneration than existing ones, a new three-dimensional Schwann cell culture technique using Matrigel and dorsal root ganglion(DRG) was developed. Nerve conduit of three-dimensionally arranged Schwann cells was fabricated using direct seeding of freshly harvested DRG into a Matrigel filled silicone tube (I.D. 1.98 mm, 14 mm length) and in vitro rafting culture for 2 weeks. The nerve regeneration efficacy of three-dimensionally cultured Schwann cell conduit (3D conduit group, n=6) was assessed using SD rat sciatic nerve defect of 10 mm, and compared with that of silicone conduit filled with Matrigel and Schwann cells prepared from the conventional plain culture method (2D conduit group, n=6). After 12 weeks, sciatic function was evaluated with sciatic function index (SFI) and gait analysis, and histomorphology of nerve conduit and the innervated tissues of sciatic nerve were examined using image analyzer and electromicroscopic methods. The SFI and ankle stance angle (ASA) in the functional evaluation were -60.1±13.9, 37.9􀆆±5.4􀆆in 3D conduit group (n=5) and -87.0 ±12.9, 32.2􀆆±4.8􀆆in 2D conduit group (n=4), respectively. And the myelinated axon was 44.91%±0.13% in 3D conduit group and 13.05%±1.95% in 2D conduit group to the sham group. In the TEM study, 3D conduit group showed more abundant myelinated nerve fibers with well organized and thickened extracellular collagen than 2D conduit group, and gastrocnemius muscle and biceps femoris tendon in 3D conduit group were less atrophied and showed decreased fibrosis with less fatty infiltration than 2D conduit group. In conclusion, new three-dimensional Schwann cell culture technique was established, and nerve conduit fabricated using this technique showed much improved nerve regeneration capacity than the silicone tube filled with Matrigel􀋓 and Schwann cells prepared from the conventional plain culture method.

키워드

Three-dimensional culture Schwann cell Peripheral nerve regeneration Dorsal root ganglion Nerve conduit