성견의 골신장술에서 골절단술시 재조합 인간 골형성 단백질-7적용에 따른 가골반응과 Osteocalcin 발현도에 대한 연구

• 영문명: EXPRESSION OF OSTEOCALCIN AND CALLUS REACTION DURING DISTRACTION OSTEOGENESIS WITH RECOMBINANT HUMAN BONE MORPHOGENIC PROTEIN-7 INJECTION
• 발행기관: 대한구강악안면외과학회
• 저자명: 조영철(Yeong-Cheol Cho) 성일용(Iel-Yong Sung) 변준호(June-Ho Byun) 박봉욱(Bong-Uk Park) 김욱규(Uk-Kyu Kim) 신상훈(Sang-Hun Shin) 김종렬(Jong-Ryoul Kim)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면외과학회지 제32권 제4호, 317~326쪽, 전체 10쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2006.08.30

영문 초록

Purpose : The aim of this study was to evaluate the effect of recombinant human bone morphogenetic protein-7 (rhBMP-7) injected on the rate of new-bone formation for distraction osteogenesis on dogs. Materials & Methods : Twelve adult dogs were randomly selected into two groups of six dogs on each. Unilateral osteotomies were performed on the body of the mandible and an intraoral distractor was mounted to the mandible on dogs. One group was treated with injection of rhBMP-7 and the other group served as the control. RhBMP-7 was administered on the day of surgery by single injection into the medullary bone at the osteotomy gap. Distraction was performed five days after osteotomy as a rate of 0.5 mm twice per day for 10 days. The animals were then sacrificed at 2, 4, and 8 weeks after completion of the distraction. Two dogs in each group, totaling four dogs, were killed at 2 weeks, 4 weeks, and 8 weeks after completion of distraction, respectively. The lengthened mandibles were harvested and processed for radiographic and histological examinations. In addition, immunohistochemical examination using osteocalcin expression was studied. Results : Radiographs showed accelerated regenerate ossification with maturation of new bone in the rhBMP-7 group comparing with the control group at the 4 weeks of the consolidation. There was no significant difference in the radiographic findings at the 2 weeks and 8 weeks of the consolidation period. Histological findings demonstrated increased bone healing pattern in the rhBMP-7-treated group during all observation period. The expression of osteocalcin immunoreactivity was hardly detected in the normal mandible of dog, but the expression was detected in all experimental rhBMP-7 treated specimens. There were also significant increasing in number of positive immunostaining cells and staining intensity of osteocalcin expression in the rhBMP-7 treated group compared with those of the control group on 2-weeks and 4-weeks. There was a significant decreasing in staining intenstiy of all both two groups on 8 weeks of consolidation period, but significant differences of immunostaining was not seen in two groups. Conclusions : A single injection of rhBMP-7 at the time of osteotomy may stimulate the rate of regenerate ossification and increase callus maturation during distraction osteogenesis. In addition, it may shorten the distraction osteogenesis procedure and decrease the prevalence of complications associated with mandibular distraction osteogenesis.

키워드

Distraction Osteogenesis rhBMP-7 Osteocalcin