골막기원세포에서 발현되는 혈관내피세포성장인자 관련 자가성장

• 영문명: VEGF-RELATED AUTOCRINE GROWTH IN PERIOSTEAL-DERIVED CELLS
• 발행기관: 대한구강악안면외과학회
• 저자명: 박봉욱(Bong-Wook Park) 이성균(Seong-Gyun Lee) 하영술(Young-Sool Hah) 김덕룡(Deok-Ryong Kim) 조영철(Yeong-Cheol Cho) 성일용(Iel-Yong Sung) 김욱규(Uk-Kyu Kim) 김종렬(Jong-Ryoul Kim) 변준호(June-Ho Byun)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면회과학회지 제35권 제5호, 294~298쪽, 전체 5쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2009.10.30

영문 초록

Purpose: The development of a microvascularization is important for the homeostasis of normal bone. Vascular endothelial growth factor (VEGF) is one of the most important factors in vessel formation. The purpose of this study was to examine VEGF-related autocrine growth in periostealderived cells. Materials and methods: Periosteal-derived cells were obtained from mandibular periosteums and introduced into the cell culture. After passage 3, the periosteal-derived cells were further cultured for 21 days in an osteogenic inductive culture medium containing dexamethasone, ascorbic acid, and β-glycerophosphate. Results: The expression of four VEGF isoforms and VEGFRs was observed in periosteal-derived cells. Treatment with cultures with VEGFR-1 and VEGFR-2 Kinase Inhibitor inhibited osteoblastic differentiation and alkaline phosphatase (ALP) activity of periosteal-derived cells. In addition, exogenous VEGF treatment increased calcium content in the periosteal-derived cells. Conclusion: These results suggest that VEGF might act as an autocrine growth molecule during osteoblastic differentiation of cultured human periosteal-derived cells.

키워드

Periosteal-derived cells Vascular endothelial growth factor Autocrine growth