Matrix metalloproteinase(MMP) sensitive hyaluronic acid hydrogel-nanoparticle complex와 rhBMP-2를 이용한 골재생

• 영문명: BONE REGENERATION WITH MMP SENSITIVE HYALURONIC ACID-BASED HYDROGEL, rhBMP-2 AND NANOPARTICLES IN RAT CALVARIAL CRITICAL SIZE DEFECT(CSD) MODEL
• 발행기관: 대한구강악안면외과학회
• 저자명: 남정훈(Jeong Hun Nam) 박종철(Jong Chul Park) 유상배(Sang Bae Yu) 정용일(Yong Il Chung) 태기융(Gi Yoong Tae) 김정주(Jung Ju Kim) 박용두(Yong Doo Park) 장정원(Jeong Won Jahng) 이종호(Jong Ho Lee)
• 간행물 정보: 『대한구강악안면외과학회지』대한구강악안면회과학회지 제35권 제3호, 137~145쪽, 전체 9쪽
• 주제분류: 의약학 > 내과학
• 파일형태: PDF
• 발행일자: 2009.06.30

영문 초록

As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase(MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel-Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.

키워드

matrix metalloproteinase(MMP) hyaluronic acid controlled release rhBMP-2 nanoparticle MMP sensitive hydrogel