당뇨 쥐에서 Vitamin B6 (PLP)의 망막혈관누출 감소와 혈관내피성장인자의 억제 효과

• 영문명: Suppression of VEGF and Decrease in Vascular Leakage by Pyridoxal 5’-Phosphate in Diabetic Rats
• 발행기관: 대한안과학회
• 저자명: 홍인환 유형곤 박성표,In Hwan Hong, MD, Hyeong Gon Yu, MD, PhD,, Sung Pyo Park, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume53,number5, 700~706쪽, 전체 7쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2012.05.15

영문 초록

Purpose: The authors of the present study investigated whether pyridoxal 5’-phosphate (PLP), an active coenzyme of vitamin B6, could inhibit the development of diabetic retinopathy in streptozotocin (STZ)-induced diabetic rats. Methods: Seven-week-old Spraque-Dawley rats (n = 20) were used in the present study. STZ (70 mg/kg) was injected intraperitoneally to induce diabetes. Blood glucose and body weight were monitored. Intraperitoneal injections of 5 μg and 50 μg PLP were administered every two days from the second week of induced diabetes. During the third week of PLP injections, the concentration level of plasma homocysteine was measured. In addition, functional status was examined by vitreous fluorophotometer and anatomical status by vascular endothelial growth factor (VEGF) staining in the retina. Results: Based on vitreous fluorophotometry examination, the PLP injection group proved to have a lower level of fluorescein concentration in the vitreous. Additionally, immunohistochemical staining revealed down-regulation of VEGF expression in the PLP group. In addition, the PLP group had a lower plasma homocysteine concentration. However, an over-dosage injection of PLP did not appear to have any noticeable impact on the treatment of diabetes. Conclusions: PLP, an active coenzyme of vitamin B6, proved to have inhibitory effects on VEGF expression and vascular leakage in the diabetic rat retina. J Korean Ophthalmol Soc 2012;53(5):700-706

키워드

Diabetic retinopathy, Pyridoxal 5’-phosphate (PLP), Vascular endothelial growth factor (VEGF), Vitreous fluorophotometry