섬유주세포에서 덱사메타존이 산화질소의 생성에 미치는 영향

• 영문명: Effect of Dexamethasone on the Production of Nitric Oxide in Trabecular Meshwork Cells
• 발행기관: 대한안과학회
• 저자명: 이수윤 김재우,Soo Yoon Lee, MD, Jae Woo Kim, MD, PhD
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume50,number8, 1254~1258쪽, 전체 5쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2009.08.15

영문 초록

Purpose: To investigate the effects of dexamethasone (DEX) on the production of nitric oxide (NO) and its enzymatic synthetic pathway in cultured human trabecular meshwork (HTM) cells. Methods: Primarily cultured HTM cells were exposed to 0, 10, 100, 1000 nM of DEX for 3 days. In addition, 100 μM sepiapterin, 100 μM ascorbic acid, and 10 μM methotrexate were co-exposed to DEX. The cellular survival and nitrite production rates were assessed by MTT assay and Griess assay, respectively. Results: DEX did not significantly affect the survival of cultured HTM cells. DEX decreased the NO production in a dosedependent manner. With co-exposure of DEX, ascorbic acid ified the DEX-induced decrease of NO production. Sepiapterin and methotrexate did not affect DEX-induced decrease of NO production. Conclusions: DEX decreased NO production in HTM cells and the de novo pathway of tetrahydrobiopterin may be involved. This decrease may raise intraocular pressure by decreasing trabecular outflow.

키워드

Dexamethasone, Nitric oxide, Tetrahydrobiopterin, Trabecular meshwork cells