원추각막에서 발현된 유전자 연구

• 영문명: Idenfitied Differentially Expressed Genes in Keratoconus
• 발행기관: 대한안과학회
• 저자명: 이지은 엄부섭 이종수,Ji Eun Lee, M.D., Boo Sup Oum, M.D., Jong Soo Lee, M.D.
• 간행물 정보: 『대한안과학회지』Ophthalmological Society,volume47,number12, 2012~2019쪽, 전체 8쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2006.12.31

영문 초록

Purpose: To evaluate the pathogenesis of keratoconus through differentially expressed genes in human keratocyte of keratoconus. Methods: Total RNAs extracted from primary cultured keratocytes in normal cornea and keratoconus were used for the synthesis of cDNA. Differentially expressed genes were screened by ACP-based PCR method using GeneFishingTM DEG kits. The differentially expressed bands were sequenced and analyzed, and identified genes were further evaluated by RT-PCR. Results: Over-expressions of BMP4 and CFL1 and under-expression of CFL1, GRCC10, and TIMP3 were verified, and comfirmed by RT-PCR. All confirmed genes were concerned with cytoskeleton, wound healing, and apoptosis. Conclusions: The identified differentially expressed genes seem to be important role in the mechanism of keratoconus, and apoptosis as well as cytoskeleton and wound healing may be attributed to the underlying stromal keratocyte.

키워드

Differentially expressed genes (DEGs), GeneFishTM PCR, Keratoconus