갑상선질환에서 Sonic Hedgehog 단백질 발현의 임상적 의의

• 영문명: Sonic Hedgehog Protein Expression in Various Thyroid Tissues and Its Clinical Implication
• 발행기관: 대한내분비외과학회
• 저자명: 유근수 이옥준¹ 김원재² 박성수 김동주 박진우 최재운 장이찬 OrloH.Clark³ Kuhn-Soo Ryu M.D. Ok-Jun Lee M.D.¹ Wun-Jae Kim M.D.² Sung Su Park M.D. Dong-Ju Kim M.D. Jin- Woo Park M.D. Jae-Woon Choi M.D. Lee-Chan Jang M.D. and Orlo H. Clark M.D.³
• 간행물 정보: 『The Koreran journal of Endocrine Surgery』11권4호, 234~241쪽, 전체 8쪽
• 주제분류: 의약학 > 일반외과학
• 파일형태: PDF
• 발행일자: 2011.12.30

영문 초록

Purpose: The Hedgehog (Hh) signaling pathway is important in embryonic development including cell differentiation and proliferation. Recently, activation of this pathway has been implicated in several forms of solid cancers. We investigated sonic hedgehog (Shh) protein expression and its relation to differentiation and clinicopathologic characteristics in thyroid cancer cell lines and tissues. Methods: We used five thyroid cancer cell lines: TPC-1, FTC-133, FTC-236, FTC-238, and XTC-1. We made tissue microarray slides using 80 thyroid surgical specimen: 40 benign and 40 malignant lesions. Immunohistochemical staining was performed using anti-Shh antibody. mRNA expression of NIS, thyroglobulin, and CD97 were evaluated by RT-PCR. Cyclopamine was used as a Shh signal inhibitor. Results: Shh expression was more prominent in TPC-1, FTC-133, and XTC-1 cell lines than the others. Cyclopamine downregulated CD97 and upregulated thyroglobulin mRNA expression, but did not induce mRNA expression of NIS. Thyroid tissues showed varied expression of Shh in both benign and malignant diseases. Shh expression was detected in 38 of 50 (76%) normal, in 18 of 25 (72%) non-neoplastic benign, in nine of 15 (60%) benign tumors, and in 31 of 40 (77%) malignant tumors. Shh over-expression was significantly less frequent in papillary thyroid carcinomas than in normal or benign thyroid tissues. In addition, Shh protein expression did not relate to clinicopathologic characteristics in papillary thyroid carcinomas. Conclusion: Thyroid tissues and cell lines vary in expression of Shh. Cyclopamine can induce redifferentiation in thyroid cancer cell lines. Shh protein expression, however, is unrelated to clinicopathologic characteristics in papillary thyroid carcinomas. (Korean J Endocrine Surg 2011;11:234-241)

키워드

Sonic hedghog, Cyclopamine, 갑상선암, 재분화,Sonic hedghog, Cyclopamine, Thyroid cancer, Redifferentiation