Absence Expression of Heat Shock Protein 25 in the Cerebellum of Ataxic Mutant Pogo Mouse

• 발행기관: 대한해부학회
• 저자명: Nam-Seob Lee Chul-Tae Kim Seung-Hyuk Chung Jin-Seong Kim Takanori Miki Yoshiki Takeuchi Young-Gil Jeong
• 간행물 정보: 『대한해부학회지』제42권 제4호, 213~218쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2009.12.31

영문 초록

Heat shock proteins (Hsps) are generally known to be induced in response to a range of stressful stimuli such as hyperthermia, immobilization, UV radiation, arsenite, arsenite, various chemicals, and drugs. In addition, these proteins have been suggested to have roles in protecting cells against apoptotic cell death. The ataxic mutant Pogo(pogo/pogo) mouse is a novel neurological ataxic mutant, which is derived from Korean wild type mouse(KJRIMskist) strain. Pogo mutation is considered as an alleles of α subunit of P/Q-type calcium channel mutants such as rolling mouse Nagoya (RMN), tottering, and leaner. We investigated the topographical Hsp2.5 expression using immunohisto-chemistry and western blot analysis in several ataxic mutant mice: RMN, tottering, leaner, Pogo and Korean wild mouse. In the cerebellum of the RMN, tottering, leaner, and normal mouse including Balb/C, C57BL/6 and ICR mouse, Hsp25 was expressed in a subset of Purkinje cells that form parasagittal stripes. The Hsp25 expression is largely -restricted to specific cerebellar lobules: VI/VII(the central zone:CZ), and IX/X (the nodular zone: NZ). Surprisingly, no Hsp25-immunoreactive Purkinje cells were seen in CZ and NZ of the cerebellum of Pogo(pogo/pogo), heterozygotes Pogo(pogo/+), and Korean wild mouse. Moreover, in western blot analysis, there was no cerebellar Hsp25 expression in ataxic Pogo mouse including Korean wild mouse. These data suggest that cerebellar Hsp25 expression was irrelevent with the development of ataxia in Pogo mouse.

키워드

Hsp25 Pogo mouse Cerebellum