차나무 기내 배양을 위한 消毒 藥劑 選拔

• 영문명: Selection for disinfectant shoot of tea plants grown in vitro culture conditions.
• 발행기관: 한국차학회
• 저자명: 김주희(J.H, Kim) 김정운(J.U, Kim) 신길호(G.H, Shin) 최형국(H.K, Choi) 김선우(S.W, Kim)
• 간행물 정보: 『한국차학회지』제3권 제2호, 135~139쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.12.30

영문 초록

For cutting in vitro, culture conditions of tea plants, monthly contamination rate, selection of disinfectants and infectant exposure time, were investigated. Monthly contamination rates were 12% in May, 15% in Feburuary, 32% in November and 37% in September in vitro conditions. Therefore, it was considered that optimum cutting time was from March to April. The disinfectant of 95% ethanol and 7--8% CaOCl treated was heavily contaminated by 8~9% than 95% ethanol and 25% NaOCl treated by 3% but in the latter case, survival rate was reduced and the disinfective degree was reduced in low concentration. Contamination rates on disinfectant exposure time were 10~16% in the 95% ethanol and CaOCl treated for 15~20min and decreased by 9~11% in the 95% ethanol and 25% NaOCl for 15~20min. but necrosis rate of the latter was 18~79% and survival rate was reduced. The optimum disinfection conditions in vitro culture tea plants are as follows ; after dip plant in the 95% ethanol for 3~5 seconds, disinfect by 7~8% CaOCl treated for 15~20min. and rinse in sterilized water, 3~5 time repeatly.

키워드

tea plant in vitro culture contamination CaOCl NaOCl ethanol