골격근에서의 H₂O₂에 의한 세포사멸

• 영문명: Hydrogen peroxide induces apoptosis in mouse skeletal muscle C2C12 muscle
• 발행기관: 고려대학교 보건과학연구소
• 저자명: 김창주(ChangJu Kim) 정운원(WoonUn Jung) 이승관(SeungGwan Lee) 이창규(ChangKyou Lee)
• 간행물 정보: 『보건과학논집』保健科學論集 Vol.29 No.2, 57~65쪽, 전체 9쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2003.12.01

영문 초록

  Reactive oxygen species (ROS) have been implicated in the pathogenesis of various diseases, and play an important role in the skeletal muscle damage through strenuous exercise or its aging. To investigate whether H₂O₂, as main precursor of highly reactive free radicals, induces apoptosis in mouse skeletal muscle cells C2C12, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide(MTT) assay, terminal deoxynuclotidyl transferase -mediated dUTP nick end-labeling (TUNEL) assay, 4,6-diamidino-2- phenylindole (DAPI) staining, DNA fragmentation assay, reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis were performed. Through morphological and biochemical analyses, it was demonstrated that C2C12 cells treated with H₂O₂ exhibit classical apoptotic features. In addition, it was shown that H₂O₂ induces increases in the levels of bax, and a decrease in the bcl-2 expression. Based on the results, H₂O₂ appears to activate specific intracellular death-related pathways and the induction of apoptosis in skeletal muscle cells.

목차

Abstract
Ⅰ. 서론
Ⅱ. 대상 및 방법
Ⅲ. 결과
Ⅳ. 고찰
Ⅴ. 결론
참고문헌