Effect of silver nanoparticles and antioxidants on micropropagation of Rosa hybrida ‘Sahara’ via nodal culture

• 발행기관: (사)한국식물생명공학회
• 저자명: Jung Won Shin Sejin Kim Jin Hyun Choi Chang Kil Kim
• 간행물 정보: 『Journal of Plant Biotechnology』51권 3호, 219~227쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2024.09.01

국문 초록

Excessive ethylene production in rose tissue culture represents a major challenge that impacts rose health and cultivation. We aimed to investigate the effects of silver nitrate (AgNO3), silver nanoparticles (AgNPs), and antioxidants on plant growth and ethylene production to establish an in vitro growth method for Korean-cultivated Rosa hybrida ‘Sahara.’ Nodal explant cultures of shoots were grown in a medium containing AgNPs, AgNO3, or antioxidants (ascorbic acid, citric acid, or both). We assessed the impact on growth, ethylene production, reactive oxygen species levels, and expression of genes associated with ethylene biosynthesis and signal transduction. Addition of AgNPs and AgNO3 to the medium mitigated overhydration and chlorosis, leading to improved SPAD values, fresh weight, and growth parameters compared to those of the control. Superoxide anion levels in the AgNP treatment group were lower than in all other treatment groups (p < 0.05). Ethylene concentrations and ethylene biosynthetic gene expression levels were significantly lower in the AgNP and AgNO3 treatment groups than in the control group (p < 0.05). In contrast, antioxidant treatments showed significant effects. Therefore, AgNPs may be suitable for enhancing the quality of plantlets in rose tissue culture by mitigating ethylene production-related challenges.

목차

Introduction

Materials and Methods

Results

Discussion

Conclusion

References

키워드

Silver nanoparticles Antioxidants Ethylene production in vitro micropropagation Nodal culture Rosa hybrida