Physiological and molecular analysis of OsTPS30 by gamma irradiation

• 발행기관: (사)한국식물생명공학회
• 저자명: Se Won Kim In Jung Jung Sang Hoon Kim Hong-Il Choi Si-Yong Kang Jin-Baek Kim
• 간행물 정보: 『Journal of Plant Biotechnology』46권 2호, 88~96쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.06.30

영문 초록

Terpenes constitute a large class of secondary metabolites in plants. The Oryza sativa terpene synthase is a vital gene in plant defense response. In this study, the molecular and physiological functions of Oryza sativa terpene synthase 30 (OsTPS30, LOC_Os08g07080) were investigated after exposure of the seeds and plants to gamma-rays. The OsTPS30 expression was slightly induced at 200 Gray (Gy), but was significantly induced at 400 Gy. The total terpenoid was synthesized more in OsTPS30- overexpressing (OX-OsTPS30) Arabidopsis thaliana plants than in wild-type (WT) plants. The OX-OsTPS30 plants exhibited resistance to gamma-rays, as compared to WT. The OX-OsTPS30 plants had significantly increased height and weight after gamma irradiation. Additionally, the activity of antioxidant enzymes was increased more in OX OsTPS30 plants than in WT plants after gamma irradiation. Furthermore, the OsTPS30-GFP fusion protein was mostly localized in the chloroplast, suggesting that OsTPS30 is putative MEP pathway-related terpene synthase.

목차

Introduction

Materials and methods

Results

Discussion

키워드

Terpene synthase gamma-ray chloroplast MEP pathway