백합나무의 체세포배 유도에 미치는 ABA, 환원질소원 및 삼투압제 효과

• 영문명: Effects of ABA, reduced nitrogen source and osmoticum for somatic embryogenesis in Liriodendron tulipifera
• 발행기관: (사)한국식물생명공학회
• 저자명: 김용욱(Yong Wook Kim) 한무석(Mu Seok Han) 문흥규(Heung Kyu Moon) 박소영(So Young Park)
• 간행물 정보: 『Journal of Plant Biotechnology』38권 2호, 186~190쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.06.30

영문 초록

This study was conducted to evaluate effects of various kinds or concentrations in abscisic acid (ABA), reduced nitrogen sources (casein hydrolysate, casamino acid and L-glutamine) and osmoticum for production of somatic embryos (SEs) from pro-embryogenic mass (PEM) in yellow poplar (Liriodendron tulipifera). In comparison of various concentrations of ABA, the highest number (640/10 mg PEM) of SEs was marked in the treatment of 0.5 mg/L. With higher concentration than 0.5 mg/L ABA, number of induced SEs were decreased. And the lowest number of SEs were obtained from the treatment of 20 mg/L ABA. Differences of 8 treatments of the nitrogen sources in the medium were also compared. In the experiment of 8 treatments for SEs production, the highest result showed in the treatment of 500 mg/L casamino acid (223/5 mg PEM). In comparison of different kinds/concentrations of osmotica for SEs induction, the best response was obtained from the treatment of 4% sucrose (317/5 mg PEM). In contrast, no SEs were found from the treatments supplemented with any concentrations of maltose.

키워드

abscisic acid nitrogen sources osmotica somatic embryo yellow poplar