무스카리 ‘Early Giant’ 잎 절편 유래 캘러스 배양을 통한 고빈도 체세포배 발생

• 영문명: High frequency somatic embryogenesis through leaf explant-derived callus culture in Muscari armeniacum cv. ‘Early Giant’
• 발행기관: (사)한국식물생명공학회
• 저자명: 이향분 (Hyang Bun Lee) 전수민(Su Min Jeon) 정미영(Mi Young Chung) 한증술(Jeung Sul Han) 김창길(Chang Kil Kim) 임기병(K
• 간행물 정보: 『Journal of Plant Biotechnology』39권 1호, 69~74쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.03.31

영문 초록

Using calli of Muscari armeniacum cv. ``Early Giant`` that is monocotyledonous ornamental bulb crop with increasing demand in Korea, we carried out current studies to establish an in vitro multiple propagation protocol via somatic embryogenesis. We found that soft pale yellow green calli were induced from leaf explants cultured on all media containing 0.1~3.0 mg·L-1 auxins such as 1-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). However, induced calli showed vigorous growth only when they further transferred on same media containing 2,4-D, 4-amino-3,5,6-tri-chloropicolinic acid (picloram), or 3,6-dichloro-o-anisic acid (dicamba). Although frequency of somatic embryo induction depended on callus source and PGR composition in somatic embryo induction media, somatic embryogenesis was initiated on surface of proliferated calli after transferring on media with no PGR or 0.01 mg·L-1 NAA co-supplemented with various cytokinins such as N6-benzylaminopurine (BAP). Highest number of embryo at 9.3 per callus clump was obtained when calli which were grown under 0.1 mg·L-1 picloram supplementation were sub-cultured on medium with 0.01 mg·L-1 NAA and 0.5 mg·L-1 BAP. In addition, morphological characteristics of somatic embryo were categorized into following nine phases: globular, biased heart, biased torpedo, early cotyledonary, middle cotyledonary, late cotyledonary, early sprouting, middle sprouting, and late sprouting embryos.

키워드

monocotyledon plant growth regulator regeneration somatic embryogenesis sprouting embryo