소나무의 체세포배 유도 및 발아에 미치는 ABA, 삼투압제 및 배발생조직 라인 효과

• 영문명: Effects of ABA, osmoticum and embryogenic tissue Lines for somatic embryo induction and germination in Pinus densiflora
• 발행기관: (사)한국식물생명공학회
• 저자명: 김용욱(Yong Wook Kim) 문흥규(Heung Kyu Moon)
• 간행물 정보: 『Journal of Plant Biotechnology』39권 2호, 93~98쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.06.30

영문 초록

This study was conducted to evaluate effects of somatic embryos (SEs) induction with different kinds and concentrations of osmoticum, with gelrite and SEs germination with different abscisic acid (ABA) and embryogenic tissue lines (ETLs) in Japanese red pine (Pinus densiflora). In comparison of somatic embryos induction with different kinds and concentrations of osmoticum, the highest record (45/90 mg FW) was obtained from the treatment of 0.1 M maltose+3.75% PEG 4000. In addition, the higher one was also recorded from 0.2 M maltose (41 SEs), it turned out this treatment was also effective in induction of SEs with previous one. In effects of various gelrite concentrations for SEs production, no SEs were occurred in the treatment of 0.4 or 0.6% gelrite, however, the highest no. of SEs shown in 1.0% gellrite (41 SEs), and some comparable results were also marked with 0.8 (37.3 SEs) or 1.2% (39.7 SEs) gelrite. Therefore, SEs can be produced from above 0.8% concentration of gelrite. Finally, in comparison of SEs germination with different concentrations of ABA, ETLs, and AC, the best germination rates (45%) were obtained from the SEs derived from both concentrations of 150 and 200 μM ABA in 06-6 ETL and when cultured on germination supplemented with 0.2% AC. When SEs were cultured on germination medium without AC, best germination rate (28.9%) came from the SEs which cultured on 250 μM ABA in 06-6 ETL

키워드

ABA embryogenic tissue Line Japanese red pine somatic embryo gelrite germination osmoticum