벼의 small ubiquitin-Like modifier E3 Ligase, OsSIZI 들연변이체의 특성 분석

• 영문명: Chanictetizafion of small ubiquifin-Like modifier E3 Ligase, OsSIZ1, mutant in rice
• 발행기관: (사)한국식물생명공학회
• 저자명: 박형철(Hyeong Cheol Park) 구성철(Sung Cheol Koo) 김훈(Hun Kim) 최원균(Won Kyum Chol) 윤대진(Dae Jin Yun)
• 간행물 정보: 『Journal of Plant Biotechnology』39권 4호, 235~241쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2012.12.31

영문 초록

Sumoylation is a reversible conjugation process that attaches the small ubiquitin modifier (SUMO) peptide to target proteins and regulates a wide variety of cellular functions in eucaryotes. As final step of the sumoylation, SUMO E3 ligases facilitate conjugation of SUMO to target proteins. To characterize the functions of the SUMO E3 ligases in Oryza saliva, we isolated a single recessive rice SUMO E3 ligase, Ossizl-2 mutant. In addition, we also conflirmed the interaction between OsSTZ1/-2 and OsSUMO1, respectively, by using an Agrobacterium-based tobacco luciferase transient expression system. Ossizl-2 mutant exhibited approximately 20% reduction in growth and developmental units compared with wild type. Especially, number of filled seeds and total seed weight were dramatically decreased in the Ossizl-2 mutant rice. Thus, these results suggest that sumoylation by the OsSIZ1 as SUMO E3 ligase plays an important role in regulating growth and development in rice.

키워드

Agronomic traits Rice SIZIPIAS-type SUMO E3 Ligase SUMO sumoylation