무선발 형질전환 Bt벼의 도입유전자 구조 분석

• 영문명: Transgene structures of marker-free transgenic Bt rice plants
• 발행기관: (사)한국식물생명공학회
• 저자명: 조현석(Hyun Suk Cho) 임명호(Myung Ho Lim) 이승범(Seung Bum Lee) 우희종(Hee Jong Woo) 신공식(Kong Sik Shin) 이진형(Jin
• 간행물 정보: 『Journal of Plant Biotechnology』40권 3호, 135~140쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2013.09.30

영문 초록

A less simple approach developed for generation of marker-free transgenic plants is to select transformants without the use of selective marker genes. Some results about development of marker-free transgenic plants were obtained using a non-selective approach in several crops such as rice, potato and tobacco. However, the study did not provide evidence on detailed characterization of introduced gene on genome, a critical step for confirming the stable integration and transmission of a foreign gene. In this study, we evaluated structure and integration sites of transgene (mCry1Ac) in the transgenic Bt rice plants which were made via conventional Agrobacterium-mediated transformation by non-selective method. Structure and integration sites of transgene in these transgenic plants had similar fashion as those recovered under selection.

키워드

Non-selective Marker-free transgenic rice Agrobacterium-mediated transformation Transgene integration site