Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

• 발행기관: (사)한국식물생명공학회
• 저자명: Hyun A Kim Suk Yoon Kwon Moon Sik Yang Pil Son Choi
• 간행물 정보: 『Journal of Plant Biotechnology』41권 1호, 50~55쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2014.03.31

영문 초록

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

목차

Introduction

Plant materials and methods

Results and discussion

Acknowledgment

References

키워드

Dengue virus Edible vaccine Immature embryo Maize transformation Paromomycin