Production of transgenic cucumber expressing phytoene synthase-2A carotene desaturase gene

• 발행기관: (사)한국식물생명공학회
• 저자명: Hyun A Jang Setyo Dwi Utomo Suk Yoon Kwon Sun-Hwa Ha Ye Xing-guo Pil Son Choi
• 간행물 정보: 『Journal of Plant Biotechnology』43권 3호, 341~346쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2016.09.30

영문 초록

The objectives of this study were to 1) evaluate the efficiency of the protocol of Agrobacterium-mediated transformation of cucumber to introduce phytoene synthase-2a carotene desaturase (PAC genes); 2) demonstrate the integration of PAC genes into the genome of putative transgenic cucumber based on growth on selection medium, PCR and Southern analysis; 3) evaluate the expression of PAC genes in transgenic cucumber based on the analysis of RT-PCR and Northern blot hybridization. Out of 5,945 cotyledonary-node explants inoculated with Agrobacterium, 65 (1.1%) explants produced 238 shoots. Integration of PAC genes into the genome of the cucumber was demonstrated based on the analysis of gDNAPCR, 21 out of the 238 plants regenerated; while 6 plants proved positive for Southern blot hybridization. Transgene expression was demonstrated based on analysis of RT-PCR, 6 plants proved positive out of the 6 plants analyzed; while 4 plants out of 6 proved positive during Northern blot hybridization. This study successfully demonstrated the production of transgenic cucumber, integration, and expression of the PAC gene in cucumber.

목차

Introduction

Materials and Methods

Results and Discussion

Acknowledgments

References

키워드

β-carotene Cucumis sativus cotyledonary-node explant transformation efficiency