감미단백질 관련 브라제인, 타우마틴 및 미라쿨린 유전자를 이용한 형질전환 상추 육성 및 발현분석

• 영문명: Stable expression and characterization of brazzein, thaumatin and miraculin genes related to sweet protein in transgenic lettuce
• 발행기관: (사)한국식물생명공학회
• 저자명: 정여진(Yeo Jin Jung) 강권규(Kwon Kyoo Kang)
• 간행물 정보: 『Journal of Plant Biotechnology』45권 3호, 257~265쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2018.09.30

영문 초록

Sweetener is one of the additives that makes you feel sweet. Artificial sweeteners and sugar are typical examples, and sweetness proteins with sweetness characteristics have been widely studied. These studies elucidated the transformation lettuce cells with Agrobacterium method for stable production of natural sweet proteins, brazzein, thaumatin, and miraculin. In this paper, we report use of a plant expression system for production of sweet proteins. A synthetic gene encoding sweet proteins was placed under the control of constitutive promoters and transferred to lettuce. High and genetically stable expression of sweetener was confirmed in leaves by RT-PCR and Western blot analysis. Sweet proteins expressed in transgenic lettuce had sweetnessinducing activity. Results demonstrate recombinant sweet proteins correctly processed in transgenic lettuce plants, and that this production system could be a viable alternative to production from the native plant.

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키워드

Taste modifying protein Brazzein Thaumatin Miraculin Transgenic lettuce Agrobacterium method