Effect of nitrogen sources and 2, 4-D treatment on indirect regeneration of ginger (Zingiber officinale Rosc.) using leaf base explants

• 발행기관: (사)한국식물생명공학회
• 저자명: Valiyaparambath Musfir Mehaboob Kunnampalli Faizal Palusamy Raja Ganesan Thiagu Abubakker Aslam Appa
• 간행물 정보: 『Journal of Plant Biotechnology』46권 1호, 17~21쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.03.31

영문 초록

Ginger is an important monocotyledonous plant belonging to the family Zingiberaceae. The objective of this study was to investigate the regeneration potential of ginger using leaf base explants. Auxins such as 2, 4-D and NAA in combination with BA were used for initiation of callus. Different combinations of both ammonium (NH4+) and nitrate (NO3-) were also studied for efficient callus production. High frequency of white friable calli was observed on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2, 4-D, 0.5 mg/L NAA and 0.5 mg/L BA. The highest shoot induction (92.33%), shootlets number (7.33±0.33) and length (88.33±4.40) mm were achieved on MS media containing 0.5 mg/L BA. Regenerated shoots were transferred to in vitro rooting media containing 1.0 mg/L IBA. Afterwards, plantlets with well-developed root and shoot system were subjected to a twostep hardening process. 71% of plantlets survived after secondary hardening without any abnormal morphology.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Reference

키워드

Ginger Callus Ammonium Nitrate BA Hardening