Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate

• 발행기관: 대한생리학회-대한약리학회
• 저자명: Ho Jong Ra Mi Young Oh Hee Ju Kim Seung Yong Lee Dae Woon Eom Suk Kyu Lee Su-Nam Kim Kyu Sung Chung
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제22권 제2호, 163~172쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2018.03.31

영문 초록

PRF001 is a fragmented DNA polymer extracted from the testes of salmon. The purpose of this study was to assess the anti-inflammatory effect of PRF001 in itro as well as the protective effect of PRF001 intake against arthritis in a rat model. In vitro, cell survival and inflammatory markers after H2O2 treatment to induce cell damage were investigated in CHON-001 cells treated with different concentrations of PRF001. In vivo, osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA) into the knee joints of rats. After consumption of PRF001(10, 50, or 100 mg/kg) for 4 weeks, inflammatory mediators and cytokines in articular cartilage were investigated. In vitro, the levels of inflammatory markers, IL-1β, TNF-α, COX-2, iNOS, and PGE2, were significantly suppressed by PRF001 treatment. In vivo, the inflammatory mediators and cytokines, IL-1β, p- Erk1/2, NF-kB, TNF-α, COX-2, and PGE2, as well as MMP3 and MMP7, which have catabolic activity in chondrocytes, were decreased in the MIA-induced osteoarthritic rats following intake of PRF001. Histological analysis revealed that PRF001 had a protective effect on the articular cartilage. Altogether, these results demonstrated that the anti-inflammatory property of PRF001 contributes to its protective effects in osteoarthritis through deregulating IL-1β, TNF-α, and subsequent signals, such as p-Erk1/2, NF-κB, COX-2, PGE2, and MMPs.

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INTRODUCTION

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DISCUSSION

ACKNOWLEDGEMENTS

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REFERENCES

키워드

Chondrocyte Cytokine Inflammation Osteoarthritis Polydeoxyribonucleotide PRF001