청국장의 항염증 및 장점막 투과성 개선 효과

• 영문명: Effects of Fermented Soybean upon Anti.inflammation and Intestinal Mucous Membrane Permeability
• 발행기관: 한방비만학회
• 저자명: 김형구(Hyung Gu Kim) 이명종(Myeong Joo Lee) 김호준(Ho Jun Kim) 김기철(Ki Cheol Kim) Shambhunath Bose
• 간행물 정보: 『대한한방비만학회지』제12권 1호, 33~47쪽, 전체 15쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2012.06.30

영문 초록

This study was designed to investigate the effects of fermented soybean upon anti-inflammation, cytotoxicity, antioxidant and intestinal mucous membrane permeability by measuring the cell viability, NO (nitric oxide) production, DPPH, Polyphenol, HRP and TEER in cells like Raw 264.7 and HCT 116 using fermented soybean. Methods Raw 264.7 cell and HCT 166 cell were used in this study. And fermented soybean powders were used for the experimental group and soybean powders for the control group. There was inflammation response upon using lipopolysaccharide(LPS). Fermented soybean powders and soybean powders were in a respectively different dose added to the cells with LPS. MTT assay, NO, DPPH and Polyphenol measurement, TEER, HRP were conducted for each cell. The results of this study were presented in mean and standard deviation. Results 1. In Raw 254.7 cells added with 100μl/ml unfermented soybean powders, 104.95% higher than 62.59% was measured. In Raw 254.7 cells added with 100μl/ml fermented soybean powders, there was 74.90% measured higher than 62.59%, which was a significant result. 2. By a gradual increase of unfermented soybean powders like 0.1μl/ml, 1.0μl/ml, 10μl/ml, 100μl/ml, the measured NO were also gradually decreased 53.12μM, 47.57μM, 37.02μM, 28.16μM. In case of cells added with fermented soybean powders, 43.95μM NO was measured in 0.1μl/ml which is significant, and in other cases, mostly measured over 56.72μM. 3. It was inferred that fermented soybean powders have anti-inflammatory effects of maintaining intestinal mucous membrane permeability because the measured values of cells in both groups were all higher than 133.62Ω measured of cells added with only LPS. And measured values of cells in both groups were all lower than 2.26 measured of cells added with only LPS. 4. In case of experiment DPPH and polyphenol measurement, fermented group was all higher than unfermented group. Conclusion From the results of conducting MTT assay, NO measurement, and TEER, HRP by using cells Raw 264.7 and HCT-116, even though there was no significance in the correlation between cytotoxicity, anti-inflammatory effects, both unfermented soybean powders and fermented soybean powders were shown to have intestinal mucous membrane permeability improvement effects. This effects could be applicable for autoimmune diseases, chronic inflammatory diseases and so additional studies are expected in the future. From the results of conducting DPPH, Polyphenol measurement, Fermented soybean may be useful as potential antioxidant.

목차

Ⅰ. 서 론

Ⅱ. 재료 및 방법

Ⅲ. 결 과

Ⅳ. 고 찰

Ⅴ. 결 론

Ⅵ. 참고문헌

키워드

fermented soybean unfermented soybean Bacillus subtilis inflammatory intestinal mucous membrane permeability antioxidant