3T3-L1 전지방세포에서 연잎-연근 혼합 발효물의 지방세포 분화 억제 효과

• 영문명: Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes
• 발행기관: 한방비만학회
• 저자명: 이신지(Sin Ji Lee) Shambhunath Bose 이수진(Su Jin Lee) 정지은(Ji Eun Jeong) 구병수(Byung Soo Koo) 김동일(Dong Il Ki
• 간행물 정보: 『대한한방비만학회지』제13권 2호, 74~83쪽, 전체 10쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2013.12.31

영문 초록

This study was performed to evaluate the effects of fermented lotus extracts on the inhibition of differentiation in 3T3-L1 preadipocytes. Methods: Extracts of lotus leaf and lotus root were fermented using 4 different probiotics separately, including Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium breve, and Bifidobacterium longum. Inhibition of preadipocyte differentiation was examined by Oil red O dye staining. Expressions of adipogenic transcription factors including CCAAT/enhancer binding proteins (C/EBP ) and peroxisome proliferators-activated receptor (PPAR ) were analyzed by real time polymerase chain reaction and Western blotting analysis. Results: Fermented lotus extracts inhibited adipogenic transcription factors by inhibiting preadipocytes differentiation. All of the groups fermented by 4 kinds of probiotics showed reduction in Oil Red O dye staining. Bifidobacterium breve showed the most effective inhibition of C/EBP . Bifidobacterium breve and Bifidobacterium longum showed the best downregulation of PPAR expressions compared with the control and the unfermented lotus group. Conclusions: Fermented lotus extracts showed significant effects on inhibition of preadipocyte differentiation in 3T3-L1 preadipocytes showing correlation with insulin sensitivity and lipid metabolism related with obesity.

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키워드

3T3-L1 preadipocyte Lotus Probiotics Fermentation Obesity Insulin resistance