Lipopolysaccharide를 처리한 RAW 264.7 세포에서 백두옹 메탄올 추출물의 항염증 효과

• 영문명: The Anti-Inflammatory Effect of Pulsatilla koreana Methanol Extract in Lipopolysaccharid-Exposed RAW 264.7 Cells
• 발행기관: 한방비만학회
• 저자명: 이귀선(Gui Sun Lee) 김두희(Doo Hee Kim) 박중현(Joong Hyun Park) 최희승(Hee Seung Choi) 허성규(Seong Kyu Heo) 차윤엽(Yu
• 간행물 정보: 『대한한방비만학회지』제17권 1호, 1~9쪽, 전체 9쪽
• 주제분류: 의약학 > 한의학
• 파일형태: PDF
• 발행일자: 2017.06.30

영문 초록

Objectives: This study was performed to identify the anti-inflammatory effect of Pulsatilla koreana (PK) methanol extract on lipopolysaccharide (LPS) induced inflammatory. Methods: There were five groups. They were control group, LPS-exposed PK methanol extract group (0 μg/ml, 10 μg/ml, 30 μg/ml, 100 μg/ml). To measure out cytotoxicity of PK, we performed the MTT assay. To evaluate the anti-inflammatory effect of PK, we examined the inflammatory mediators, such as nitric oxide (NO), prostaglandin E2 (PGE2), and proinflammatory cytokines (interleukin [IL]-1β, IL-6, tumor necrosis factor-α [TNF-α], IL-10). Results: 1. The extract of PK (∼100 μg/ml) itself did not have any cytotoxic effect in RAW 264.7 cells. 2. The concentration of plasma NO and PGE2 in PK methanol extract group showed a lower values than those of control group. 3. The concentration of plasma IL-1β, plasma IL-6, and plasma TNF-α in PK methanol extract group showed a lower values than those of control group. 4. The concentration of Plasma IL-10 in PK methanol extract groups showed higher than control group; however, these values showed no significantly different. Conclusions: According to this study, the extract of PK could be used as a protective agent against inflammation.

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키워드

Pulsatilla koreana Lipopolysaccharide RAW264.7 Anti-inflammatory effect Cytokines