백김치로부터 분리된 Limosilactobacillus fermentum 생균과 열처리 사균체의 항산화 및 항염 효과

• 영문명: Antioxidant and Anti-Inflammatory Effects of Live and Heat-Killed Limosilactobacillus fermentum isolated from Baekkimchi
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 이가영(Ga-Young Lee) 이민경(Min-Kyeong Lee) 최지우(Ji-Woo Choi) 윤혜진(Hye-Jin Youn) 조현희(Hyun-Hee Cho) 박훈(Hoon Park)
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』10권 2호, 75~87쪽, 전체 13쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2024.12.31

국문 초록

This study aimed to evaluate the probiotic properties of Limosilactobacillus fermentum SMF680 and Limosilactobacillus fermentum SMF709, which were isolated from Baekkimchi, and to assess the antioxidant and anti-inflammatory activities of live and heat-killed cells. The selected SMF680 and SMF709 strains showed similar acid resistance, bile resistance, and intestinal cell adhesion compared to Lacticaseibacillus rhamnosus GG (LGG), a commercial probiotic strain. The live and heat-killed cells of SMF680 and SMF709 did not show toxicity at a concentration of 1 mg/mL against intestinal cells (HT-29) and macrophages (RAW 264.7). The heat-killed cells of SMF680 and SMF709 showed significantly higher DPPH and ABTS radical scavenging activities as well as β-carotene bleaching inhibitory activity than those of LGG (p<0.05). Moreover, the heat-killed cells of SMF680 and SMF709 showed significantly higher ABTS radical scavenging and β-carotene bleaching inhibition activities compared to the live cells (p<0.05). The treatment of live or heat-killed cells of SMF680 and SMF709 significantly suppressed the expression of TNF-α, IL-6, iNOS and COX-2 in LPS-stimulated RAW 264.7 macrophages (p<0.05). The live and heat-killed cells of SMF680 and SMF709 exhibited superior inhibitory activities on the expression of TNF-α, IL-6, iNOS and COX-2 compared to those of LGG. In particular, the heat-killed cells of SMF709 showed the most potent anti-inflammatory effect by inhibiting IL-6, iNOS and COX-2 expression by 73.86%, 81.93%, and 65.75%, respectively. Therefore, the results of this study suggest that the heat-killed cells of L. fermentum SMF680 and L. fermentum SMF709 strains could be utilized as natural antioxidant and anti-inflammatory agents in the food, pharmaceutical, and cosmetic industries.

목차

서 론

재료 및 방법

결과 및 고찰

요 약

Acknowledgments

References

키워드

Limosilactobacillus fermentum heat-killed probiotics paraprobiotics antioxidant activity anti-inflammatory activity